Part:BBa_K1012002:Design

The TorA twin-arginine signal sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The DNA encoding the TorA signal sequence contains no illegal restriction sites with respect to standard RFC[10]. It has a single HpaI site (gtt/aac) starting at position 95.

Source

The sequence was amplified from E. coli K-12 (MG1655) genomic DNA.

References

Méjean V, Iobbi-Nivol C, Lepelletier M, Giordano G, Chippaux M, Pascal MC. (1994) TMAO anaerobic respiration in Escherichia coli: involvement of the tor operon. Molecular Microbiology 11:1169-1179.

Santini CL, Bernadac A, Zhang M, Chanal A, Ize B, Blanco C, Wu LF. (2001) Translocation of jellyfish green fluorescent protein via the Tat system of Escherichia coli and change of its periplasmic localization in response to osmotic up-shock. Journal of Biological Chemistry 276:8159-8164.

Palmer T, Berks BC. (2012) The twin-arginine translocation (Tat) protein export pathway. Nature Reviews Microbiology 10:483-496.